pspcas9 bb 2a gfp vector (Addgene inc)
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Addgene inc
pspcas9 bb 2a gfp vector
Pspcas9 Bb 2a Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspcas9+bb+2a+gfp/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/bio_rxiv__64898__2026__10__15__759098-285-7-10
Average 96 stars, based on 4125 article reviews
Pspcas9 Bb 2a Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspcas9+bb+2a+gfp/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/bio_rxiv__64898__2026__10__15__759098-285-7-10
Average 96 stars, based on 4125 article reviews
pspcas9 bb 2a gfp vector - by Bioz Stars,
2026-11
96/100 stars
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Clone Assay:Article Title: The lncRNA DAMER selectively guides m 6 A-dependent regulation of ATF4 and asparagine metabolism under nutrient stress in cancer. Article Snippet: How cancer cells couple metabolic stress sensing to orchestrate specific survival programmes is a key question.. Here we show a long non-coding RNA (lncRNA)-guided epitranscriptomic mechanism orchestrating metabolic adaptation by controlling the stability of master stress regulator ATF4.. Glucose or glutamine deprivation induces endoplasmic reticulum stress via reactive oxygen species–NRF2-dependent transcription of the lncRNA DAMER. Article Title: A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation. Article Snippet: Candidate singleguide RNAs (sgRNAs) were identified using CRISPick (RRID:SCR_025148; https://portals.broadinstitute.org/gppx/crispick/ public), targeting all common splicing variants. .. The sgRNAs were ordered as short oligonucleotides (Microsynth AG) and cloned into pSpCas9( Article Title: Alternative splicing fine-tunes modular protein interfaces to control protein localization. Article Snippet: .. First, we cloned gRNAs targeting SNX2, either previously described73 or predicted using the CRISPRscan online tool74, into the pSpCas9( Transfection:Article Title: MMRi62 induces iron depletion–driven apoptosis through a ferritin-independent mechanism Article Snippet: Cells were grown at 37 °C/5% CO 2 in DMEM (Gibco, Thermo Fisher Scientific Inc, MA, USA) supplemented with 8% Gibco Fetal Bovine Serum (FBS, certified, heat inactivated, Thermo Fisher Scientific Inc, MA, USA). .. DAOY and HD-MB03 wildtype (WT) cells were transfected using jetPEI (Polyplus, 101000053) according to the manufacturers instructions with pSpCas9( Plasmid Preparation:Article Title: MMRi62 induces iron depletion–driven apoptosis through a ferritin-independent mechanism Article Snippet: Cells were grown at 37 °C/5% CO 2 in DMEM (Gibco, Thermo Fisher Scientific Inc, MA, USA) supplemented with 8% Gibco Fetal Bovine Serum (FBS, certified, heat inactivated, Thermo Fisher Scientific Inc, MA, USA). .. DAOY and HD-MB03 wildtype (WT) cells were transfected using jetPEI (Polyplus, 101000053) according to the manufacturers instructions with pSpCas9( Article Title: A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation. Article Snippet: Candidate singleguide RNAs (sgRNAs) were identified using CRISPick (RRID:SCR_025148; https://portals.broadinstitute.org/gppx/crispick/ public), targeting all common splicing variants. .. The sgRNAs were ordered as short oligonucleotides (Microsynth AG) and cloned into pSpCas9( Article Title: Alternative splicing fine-tunes modular protein interfaces to control protein localization. Article Snippet: .. First, we cloned gRNAs targeting SNX2, either previously described73 or predicted using the CRISPRscan online tool74, into the pSpCas9( Article Title: Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation. Article Snippet: Cells expressing monoallelic HLA class I molecules represent a convenient in vitro model for studying the immunogenicity of tumor protein neoepitopes.. Such models can be generated using endogenous HLA class I-deficient cells.. Here, we describe an effective method for eliminating HLA class I expression in HEK293T cells via simultaneous CRISPR/Cas9 editing of the HLA-A, HLA-B, and HLA-C genes using a single guide RNA. CRISPR:Article Title: MMRi62 induces iron depletion–driven apoptosis through a ferritin-independent mechanism Article Snippet: Cells were grown at 37 °C/5% CO 2 in DMEM (Gibco, Thermo Fisher Scientific Inc, MA, USA) supplemented with 8% Gibco Fetal Bovine Serum (FBS, certified, heat inactivated, Thermo Fisher Scientific Inc, MA, USA). .. DAOY and HD-MB03 wildtype (WT) cells were transfected using jetPEI (Polyplus, 101000053) according to the manufacturers instructions with pSpCas9( Article Title: The pathogenic p.D1108N SORL1 variant is dominant-negative and suitable for an antisense-oligonucleotide-mediated splice-modulating therapy for Alzheimer’s disease Article Snippet: .. The SORL1 knock-out (KO) was introduced in i3N-iPSCs by CRISPR/Cas9 using the pSpCas9( Knock-Out:Article Title: The pathogenic p.D1108N SORL1 variant is dominant-negative and suitable for an antisense-oligonucleotide-mediated splice-modulating therapy for Alzheimer’s disease Article Snippet: .. The SORL1 knock-out (KO) was introduced in i3N-iPSCs by CRISPR/Cas9 using the pSpCas9( Gene Knockout:Article Title: The pathogenic p.D1108N SORL1 variant is dominant-negative and suitable for an antisense-oligonucleotide-mediated splice-modulating therapy for Alzheimer’s disease Article Snippet: .. The SORL1 knock-out (KO) was introduced in i3N-iPSCs by CRISPR/Cas9 using the pSpCas9( Construct:Article Title: The pathogenic p.D1108N SORL1 variant is dominant-negative and suitable for an antisense-oligonucleotide-mediated splice-modulating therapy for Alzheimer’s disease Article Snippet: .. The SORL1 knock-out (KO) was introduced in i3N-iPSCs by CRISPR/Cas9 using the pSpCas9( Generated:Article Title: Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation. Article Snippet: Cells expressing monoallelic HLA class I molecules represent a convenient in vitro model for studying the immunogenicity of tumor protein neoepitopes.. Such models can be generated using endogenous HLA class I-deficient cells.. Here, we describe an effective method for eliminating HLA class I expression in HEK293T cells via simultaneous CRISPR/Cas9 editing of the HLA-A, HLA-B, and HLA-C genes using a single guide RNA. Cloning:Article Title: Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation. Article Snippet: Cells expressing monoallelic HLA class I molecules represent a convenient in vitro model for studying the immunogenicity of tumor protein neoepitopes.. Such models can be generated using endogenous HLA class I-deficient cells.. Here, we describe an effective method for eliminating HLA class I expression in HEK293T cells via simultaneous CRISPR/Cas9 editing of the HLA-A, HLA-B, and HLA-C genes using a single guide RNA. Ligation:Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress Article Snippet: .. The annealed gRNA oligowas inserted into theBbsI site of pSpCas9( |
